Show Notes
This release contains only the music track from Base by Base Episode 31.
Song title: Beyond the Eight-Base Line
Original Base by Base episode: 31: Non-canonical FBN1 splicing in the 100k Genomes Project
Article metadata:
Article title: Utility of genome sequencing and group-enrichment to support splice variant interpretation in Marfan syndrome
Journal: Genetics in Medicine
DOI: 10.1016/j.gim.2025.101477
Reference: Walker S, Bunyan DJ, Thomas HB, Kesim Y, Kershaw CJ, Holloway J, Wai H, Day M, Smith CL, Hawkes G, Wood AR, Weedon MN, Blair E, Curtis SL, Fielden C, Evans J, Whittington R, Smithson SF, Cox H, Clift P, McEntagart M, Prapa M, Alsters S, Morris-Rosendahl D, Dean J, Morrison PJ, Dixit A, Sarkar A, Prescott K, Riazat Kesh LA, Tharakan R, Turner C, Ellard S, Shaw-Smith C, Fasham J, Clowes V, Holden S, Somarathi S, Mercer C, Berry I, O’Keefe RT, Banka S, Baralle D, Thomas NS, Baple EL, Taylor JC, Pagnamenta AT. Utility of genome sequencing and group-enrichment to support splice variant interpretation in Marfan syndrome. Genetics in Medicine (2025). DOI: https://doi.org/10.1016/j.gim.2025.101477
Lyrics:
Verse 1
You swore you checked the edges of the page,
Eight little steps where answers used to stay.
But deeper in the dark of quiet code,
A hidden syllable takes the wrong road.
Pre-Chorus
A whisper cuts the sentence in two,
A phantom line the cell reads through.
Bright screens, long nights, we zoom in close—
And watch the “normal” come undone.
Chorus
It’s hiding in the in-between, out past the usual sign,
A stolen splice, a borrowed beat, beyond the eight-base line.
We name the break, we trace the thread, we pull it into light,
So hearts can keep their steady time—
Tonight, tonight.
Verse 2
A cryptic doorway opens where it shouldn’t be,
A pseudoexon slips in quietly.
Frameshift thunder or a change too clean,
Either way the protein isn’t what it’s meant to be.
Bridge
Run the genome wide, let the window breathe,
Then ask the RNA what it truly believes.
If the message skips, if the message grows,
We can find the fault that no one knows.
Final Chorus
It’s hiding in the in-between, out past the usual sign,
A stolen splice, a borrowed beat, beyond the eight-base line.
We test the blood, we map the reads, we make the meaning right,
And families get their next clear step—
Tonight, tonight.
